Luminescence was detected using the Bright-Glo Luciferase Assay System (Promega) on a SpectraMax M5 plate reader (Molecular Devices). Extracting viral DNA from 4 x 109 copies of the AAV8 preparation ( nominally 5 ng ssDNA)The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Fluorescence was measured using microplate reader at an excitation wavelength of 488 nm and emission wavelength of 529 nm using Spectramax-M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Bacterial growth was carried out at 25 °C in a 200 l volume withconstantshakingat450rpm. edu, 814-863-3503. Preparation of biotin–tyramineThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 1. After the plate is read, data will appear in the plate section: 10. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. Luminescence, Fluorescence Intensity, UV-Visible Absorbance, Filtered Luminescence, BRET and FRET. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Last lab had ours for years and never had a problem with it. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. HeLa cells were seeded in a 6-well plate with standard DMEMfluorescence intensity in the supernatant was measured in a SpectraMax M5 plate - reader (Molecular Devices) at 485 nm excitation and 535 nm emission wavelengths. Product Type. Table of Contents. It controls all the plate reader functions and offers powerful data analysis. Detection modes are available in UV-Vis absorbance, fluorescence, and. Simply unpack it, plug it in, and begin your experiments. Fluorescein 0. 7. Evans blue dye in the supernatant was detected at optical density (OD) 620 nm, using a SpectraMax M5 plate-reader (Molecular Devices, Sunnyvale CA). The relative fluorescence unit (RFU) generated from the assay was proportional to the number of living cells in each well. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing a single SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. After 30 min, the plate was washed with PBS 0. Monocytes were seeded in 24 or 96 well plates at 5 × 10 5 cells/mL and differentiated to macrophages for 7 days in X-VIVO 10 (Lonza) medium supplemented with 1% human serum as previously described . To update the driver. 7°C and the bottom to 36. Learn how to use the SpectraMax M5 SpectraMax M4 SpectraMax M5 SpectraMax M5e Multi-Mode Microplate Reader, a device that can read microplates and cuvettes with. In the M2, M5 and Plus. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. 1 and slit widths of 2. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Supersedes 1. After addition of the freshly diluted substrate (50 μL/well, from Promega), bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. Imaging opens up a range of applications for cell-based assays that cannot be performed on a standard plate reader. The Infinite and Spark multimode microplate readers are modular and upgradeable. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Select the lab user profile. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Typically the system can detect absorbance, luminescence, fluorescence,. Models for In. Fluorescence, brightfield, color brightfield, and phase contrast imaging are available with the automated cell imager. Fluorescence was recorded in a Spectramax M5 plate reader at a 0. During the room temperature incubation (step 9. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. siRNA (750 pmol) was transfected into NRK-49F (1 × 10 6 cells) using a Nucleofector Kit V and the Amaxa Nucleofector. 2. 100 μl of 2% EB (Sigma-Aldrich, St. For more sample throughput, the system can be easily integrated into a full roboticCell–cell fusion levels were measured using a luciferase assay (One-glo; Promega) and measured on a Spectra Max M5 plate reader (Molecular Devices). Briefly, Nunc microtiter plates were coated overnight at 2° to 8°C with KLH (3 μg/ml; IMMUCOTHEL, biosyn Arzneimittel GmbH) prepared in PBS. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. Linear, Orbital Slow, and Orbital Fast. For a Price List, please call (858) 646-3100 ext. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Uncontrolled Copy for Training and Reference Purposes Only . Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. On microplate readers,. ThT fluorescence was measured at 444 and. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. BioTek Gen5 software is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning applications in a variety of microplates and other labware. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). Cytation 5 extends the legacy of Agilent BioTek multimode plate readers with a modular and upgradable imaging mode. For a higher . RoHS Compliant. 9. Open and turn on the laptop. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The fluorescence intensity was measured using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif) with excitation/emission at 485/530 nm. SpectraMax Readers have the most complete level of product validation and compliance. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. Top reading is usually more sensitive. Then, luminescence of samples was read in a Spectramax M5 plate reader with integration time of 5 s. Human T-cells were cultured at 1 × 10 6 /mL for 5 days with plate-bound 10 μg/mL anti-CD3 and soluble 1 μg/mL anti-CD28. A. Activation of reporter constructs was monitored using the Dual-Glo Luciferase assay (Promega) and measured in a Spectramax M5 plate reader. 9. Cell culture All cell culture reagents were from Thermo Fisher Scientific (Loughborough, UK) unless otherwise stated. 01–10 fmol/well (0. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. Copia stocks a full line of used microplate readers. 3. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. PURPOSE. Plates were incubated at 37°C for 6 hours and then equilibrated to room temperature for 30 min. Standard curves were plotted using SoftMax® Pro Software (Figure 1). : 10 Mar 09 Page 1 of 14 Setup Guide on the BioTek Instruments FLx800 Fluorescence™ Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 Select option 3, ext. The antibiotic bacitracin A has been used as an inhibitor of thiol isomerases, including ERp5, in the insulin reductase assay . Simply unpack it, plug it in, and begin your experiments. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 9. Duplicate wells were tested for each condition, and data were graphed as a percent of control in GraphPad Prism (GraphPad Software). Plate Analysis 9. Good condition cosmetically, some signs of use such as the scratches on front of panel. Application Area: Multi mode plate reader. The concentration of infectious virus in both stock. 40266 Email: drugdiscoverytech@invitrogen. The multi-mode. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. d. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. edu, 814-863-3503. harvard. 9000-0756. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. There is currently no charge for using this plate reader. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Fluorescence values were used to calculate the percent inhibition of enzyme activity relative to maximal activity, PTP4A3 in the absence of inhibitor, and maximal inhibition, PTP4A3 in the presence of 2 mM Na 3 VO 4. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The fluorescence measurements of cell experiments were conducted with a SpectraMax M5 plate reader. In general, reading fluorescence from the top is more sensitive than reading from the bottom. Published Dec 16, 2020 | Updated Dec 16, 2020. For wavelengths in the UV range above 220 nm, UV-clear plastic plates or quartz plates should be used. DH5α and BL21 (DE3) chemically competent E. 2016 Aug 2; 7(31): 50027–50042. The GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. The exact concentration of the RNA stock solution was calculated by UV absorption at 260 nm (measured by Spectramax M5 Plate Reader, Molecular Devices, San Jose, CA) with the accepted extinction coefficient of the RNA (21. 2. Page 1 of 8 . Highly selective for dsDNA over ssDNA or RNA. Temperature control range from Ambient +4º to 50ºC. Turn on Plate Reader and wait for it to complete its start-up routine. Concentrations of other cytokines were determined by using custom MSD kits and a QuickPlex SQ120 plate reader (Meso Scale Discovery,. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. both cuvette-based and microplate reader applications on the same instrument. Cell Viability Assay Using Violet Staining The cells were seeded into 24-well plates and cultured in normal or growth medium or hormone-depleted medium with the relevant treatments as. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. Chemistry Department Facilities. S. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. To monitor the aggregation kinetics, 10 μM ThT was added to a small aliquot (300 μL) of the mixture, and the fluorescence signal (λ ex = 444 nm, λ em = 485 nm) was monitored on a Spectramax M5 plate reader (Molecular Devices). S6. g. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. 2–200 pg/well). 4 fmol/well, 384-well plate)−bottom Reading Speed (Kinetic) 96 wells: 11 s 384 wells: 22 s Luminescence Wavelength Range 300−700 nm Dynamic Range > 6 decades Sensitivity. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Web Information The compact FLx800™ fluorescence reader provides high performance in 6- to 384-well microplates at an attractive price. You can use it for many applications (OD, fluorescence, luminescence). Nonspecific binding was determined by competition with 1. M5 plate reader at 595 nm. , Sunnyvale, CA, USA) at the excitation wavelength of 488 nm for carboxy-H2DCFDA and 505 nm for DHE and MitoSOX ™. To evaluate the safety of the formulas against eyes, we used the rats that were topically administrated with one drop (20 μL) of MT, MT-LIPs, 5% TAT-MT-LIPs, or 10% TAT-MT-LIPs (all at the equaled MT-concentration of 100 μM) at a. To avoid these issues and provide the best possible dynamic range between the highest and the lowest measurement values of your assay, fluorescence gain is typically adjusted on the sample with the expected highest signal output (e. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Purified fluorescent protein was diluted 15-fold into 0. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Uncontrolled Copy for Training and Reference Purposes Only . 5. 1, and the color is formed by the reaction in the microtiter plate. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 314 low lamp level warning. The cell lysate was then transferred to a white opaque 96-well plate (50 μl/well), mixed with the freshly diluted substrate (50 μl/well), and bioluminescence was immediately measured on a SpectraMax M5 plate reader (Molecular Devices) using the luminescence mode. 5" W x 17. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. 5 × 10 5 counts/fmol, thus the suitable enzyme range for bioluminescence measurement is 0. The Chemistry Department is housed in an air-conditioned building, sharing space with the Departments of Biology and Molecular Biology & Biochemistry. Specifications General -Detection mode Fluorescence, Luminescence -Read method End point, kinetic. Chirag considers the SpectraMax M5 a multi-purpose reader that. The measured bioluminescence data were expressed as mean ± SE (n = 3) and fitted with one-site binding model using the. Features. 4, step 4, will be used for analysis. 0200-7027. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact [email protected]. An equal volume of 0. If the computer is connected to the Internet: Select Search Automatically for. Molecular Devices M5 plate readers; Multiple liquid bulk dispensers (Wellmate, Multidrop Combi) Price List. The WT DENV2C and its single-point mutants L50S, L54S, L81N and I88N were. Published Dec 16, 2020 | Updated Jun 22, 2023. The drawing shows a standard microplate of 15 mm height covered with a lid. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Users can choose to process all plates within the magazine or select a specific number. STAKMAX. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. ameters by UV-Vis assays was done on a multimode SpectraMax M5 plate reader by using 96-well plates (Fisher Scientific). Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Most papers they cultured bacteria to OD600>0. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 5°C at 37°C. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. plate and push the “drawer” button (this does not need to be done before opening/exploring the software, but must be done before reading the plate, of course). 0 out of 5 $ 3,799. Information on how to reserve the plate readerBioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. 25 pM (0. The absorbance at 450 nm was subsequently recorded with SpectraMax M5 plate reader (Molecular Devices). Supernatants were collected, and the concentration of l-lactate was determined by measuring absorbance of reaction samples using the Spectra Max M5 plate reader (Molecular Devices). The inhibitory rate of ingredients was calculated as follows: Inhibition (%) = (FI of control – FI of treatment) / FI of control × 100. The EC50 values were calculated from the absorbance values vs. 3) At the bottom of the strip, click on "Show. The BioTek Cytation cell imaging multimode microplate readers offer modularity to meet today’s workflows and are upgradable to meet future workflow requirements. For dissociation measurement, competitor R3/I5 was added and bioluminescence was continuously. The luminescent signal was recorded on a SpectraMax M5 plate reader (Molecular Dy-namics). Step 1: Open the SoftMax Pro IMAP FP protocol. The reaction was monitored with a SpectraMax M5 plate reader photometer (Molecular Devices GmbH, Biberach a. Cells were seeded at a density of 1,000 cells/well (A549: 500 cells/well). SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. After that, the medium was. 17. 9. Fluorescence, luminescence, and UV-Vis absorbance multimode plate reading. The peptide concentration was. We use Spectramax M5 microplate reader in our lab. Three kits covering a wide range of dsDNA concentrations. Devices’ StakMax® Microplate Stacker in a matter of minutes and . 2. Results: In the presence of Cliradex ®, Johnson’s® Baby Shampoo, OCuSOFT Lid Scrub®were read using SpectraMax M5 plate reader (Molecular Devices, San Jose, CA, USA) with excitation of 380 nm and emission of 460 nm. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Transfection of siRNA into cells. The SpectraMax 190 reader can be integrated with Molecular . 7°C (gradient heating design). 3) At the bottom of the strip, click on "Show. Temperature can be set to 4º above ambient to 45°C (thermometer icon) on main toolbar. 3. Appropriately. 9. 0 . Following lysis, the plate was centrifuged at 500g for 3 min and supernatants transferred to a Maxisorp 96-Well Flat-Bottom Assay Plate (Nunc). Reader Documentation Version No. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 0 μM), and this response was consistent in both plate reader assays and on chip (Supplementary Figure 5d. After 48-h growth at 25 C with agitation, the optical density was recorded using a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA). Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. After the plate is read, data will appear in the plate. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch: based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. A sensitivity level of 0. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Clear Filters. 5. MOLECULAR DEVICES VMax Kinetic Microplate Reader For Sale. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. The plate was read by a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm with 620 nm as reference. [4] The IC 50 value of each drug was calculated by the Logit approach. PURPOSE. 2013): the specific activity of both enzymes was ~1. For. Top reading is usually more sensitive. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Cells were collected and fixed with precooled 95 % ethanol at 4 ºC for 12 h. The SpectraMax ® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. After the plate is read, data will appear in the plate section: 11. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 99 was required for a valid standard curve. IL-33 concentrations in the extracts were measured with a Quantikine ELISA kit (R&D Systems, Minneapolis, Minn) and read on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, Calif). 5: Schematic of the back panel of a reader. The degradation. Copia stocks a full line of used microplate readers. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. reader. Thermo Scientific Microplate Reader Verification Plates are dedicated performance verification systems helping to verify and document the key performance parameters of Thermo Scientific Multiskan FC, Multiskan Sky, Luminoskan, Fluoroskan FL, and Varioskan LUX. a validation plate, certificate of calibration, user guide, and automated SoftMax® Pro Software protocols to run validation tests. The decrease in absorbance at 340 nm was analyzed for the 200 s of the reaction, which corresponds to the initial rate of the reaction. Options include top and bottom detection, temperature control and reagent injector. How-to-use guide for the M5 plate reader and SpectraMax Software in the CMB. Protocol for Qubit DNA BR Assay. 313 reference gain check fail. 5. To determine the DAR, STRO-002 in plasma samples were captured using Streptavidin Mag Sepharose Beads (GE Healthcare) coated with biotinylated. Absorbance readings were then taken at 650 nm using a Spectramax M5 plate reader with SoftMax Pro v5. Standard curve for Quant-iT OliGreen ssDNA M5 Plate Reader Assay with 200 µl sample volume Quantification of the AAV 8-GFP-CMV viral DNA using the Quant-iT™ OliGreen® ssDNA reagent (Cat. 0 . FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. 2 software for data reduction (Molecular Devices, Sunnyvale, CA). 305 reference level saturation. PDE-lux transcriptional fusion plasmids were constructed as previously described. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. 1. Qualitative and quantitative data analysis, along with customizable exporting and reporting enable automated end-to. 91 cm x 44. BeWo cells were plated at 1×10 4 cells/well in 96-well opaque walled plates, and viability was assessed at 72H for both CT and SCT cell types as per the manufacturer’s instructions, using a Spectromax M5 plate reader (Molecular Devices, San Jose, CA, USA). com GeneBLAzer® Assay Setup Guide on theThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and. xlsx file, with or without the averages and normalizations. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. Microtiter plates (Corning 3690; 96-well half area plates) were coated overnight at. An absorbance plate reader offers higher throughput and can measure the absorbance of samples in microplates (typically 96-well or even 384-well) by sending light through each. Fluorescence measurements were performed at 30°C (S30 extract) or 37°C (PURE system) using a SpectraMax M5 Plate Reader (Molecular Devices) set to 610 nm excitation and 650 nm. Microplate automation and detection instruments for walk away operation and high quality results. 25 For direct lysis of clots by plasmin, plasmin (250nM) was added to the surface of clots and lysis monitored by theSteady-state affinity measurements were performed on an Analyst AD, Analyst HT, or SpectraMax M5 plate reader (Molecular Devices). In the closed conformation, the regulatory SH3 and SH2 domains pack against the back of the kinase domain, providing allosteric control of kinase activity. 1 or 10 Tablet PC with USB connection to instrument. Documents. 500 mm/s is the maximum speed of the extruder of AnkerMake M5 when using Fast Mode. Riss, Germany) at 330 nm and room temperature for 30 min. S. The modular platform allows upgrading to expand functionality as your laboratory workflows change. doi: 10. 0 mM of NADH per min at pH 8. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. Fluorescence Microplate Reader Comparison Chart. Viability Assay reagent (Promega). Enter Sample IDs (HPV-Type, Sample Description and Data Reference when applicable), Dilution Factors, and background information into the template. Catalog number: 165306. Kinetic measurements were done on a SpectraMax M5 plate reader (Molecular Devices) monitoring absorbance at 380 nm at 22 °C using at least three independent measurements. Certain functionalities in the table above requires specific configurations. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. 5. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. 4-liter V-8 that sends power to all four wheels via the car’s. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. ) and had a resistivity of 18. Enzyme activity from tissue homogenates was normalized to total protein content with the Bradford protein assay (Biorad). Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 18632/oncotarget. Then, absorbance was measured by a Spectra Max ™ M5 plate reader (Molecular Devices, Sunnyvale, CA). Molecular Devices Spectramax M5 Microplate Reader; Related products. View the Molecular Devices website. Multimode microplate reader equipped with latest detections like fluorescence, absorbance etc. Menu. Multiskan Verification Plate, 8. 4. † Cells in the paper scaffolds and collected in the well plates were also imaged with an Olympus IX-70 fluorescence microscope equipped with. Samples were read on a Molecular Devices SpectraMax M5 plate reader at 365-nm excitation and 450-nm emission. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. Also refer to this Multimode Reader Comparison Chart. 마이크로플레이트 리더 (Microplate Reader) 란, 물질의 광학적 성질 (흡광, 형광, 발광) 을 이용하여 Sample 내에 있는 Target 물질 (화합물) 의 양을 측정하는 분석장비 입니다. Find regional support. Fluorescein 0. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 6), turn on the M5 Plate Reader and open the “BCA Template” file 9. Microplate Readers. The sample is typically placed in a cuvette through which light is sent horizontally. . Data were expressed as % control =. Fluorescence Microplate Readers. Fluorescence Microplate Reader Comparison Chart. A solution for low volume applications. The MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-After this time, cells were stained with crystal violet, washed 3 times and lysed. 2 only) SpectraMax M-series Microplate readers. The Back Panel Figure 1. Sample Format. The optical density at 600 nm (A 600) was monitored using SpectraMax M5 plate reader (Molecular Devices) at specific time intervals. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by issuing a single The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Microplate Reader Shelf. The dual monochromator optics allow. Description Figure 1. The absorbance was read at 450 nm with 650 nm as background correction using Softmax software on M5 plate reader (Molecular Devices). Eis inhibitors were dissolved in Tris-HCl (50 mM, pH 8. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 9. All values reported represent the mean of at least three independent experiments. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 A standard spectrophotometer measures the absorbance of one sample at a time. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Plate stacker and robot integration The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Supersedes 1. You can also interpret your results using integrated data analysis software. 05 were scored as. SpectraTest® Validation Plates User Manual & Protocol Download Page. The optical density (OD) at 450 nm was recorded by SpectraMax M5 plate reader (Molecular Devices, CA). 6 for further experiment, so I'm confused which i would choose for OD value determination.